Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock
نویسندگان
چکیده
منابع مشابه
The Current Problem : Koi Herpesvirus (khv)
Koi herpesvirus (KHV) is a pathogen agent that predominantly affects common and koi carp (Cyprinus carpio and Cyprinus carpio koi, respectively) causing disease and mass mortality in farmed and wild populations of these breeds. Following the first reports of KHV in Israel and Germany in 1998, the geographical range of this disease has become extensive. The disease has been spread to many countr...
متن کاملImmune Response to Koi Herpesvirus (KHV) of Koi and Koi × Red Common Carp (Cyprinus carpio)
Koi herpesvirus (KHV), also known as Cyprinid herpes virus 3 (Cyprinid 3) is lethal disease in common carp and koi (Cyprinus carpio). Two different groups (KK and RK) were infected KHV by intraperitoneal injection. Fish for gene expression analysis were sampled at 0 h, 12 h, 24 h, 48 h and 72 h post infection (p.i). The results showed that two immune related gene, Interferons (INFs) ɑβ and Inte...
متن کاملDetection of novel strains of cyprinid herpesvirus closely related to koi herpesvirus.
Cyprinid herpesvirus 3 (CyHV-3) or koi herpesvirus (KHV) is a devastating virus of carp. Using generic primers for the DNA polymerase and the major capsid protein genes of cyprinid herpesviruses, nucleotide sequences divergent from previously described CyHV-3 were obtained. At least 3 novel groups of putative CyHV-3-like viruses were identified, sharing 95 to 98% nucleotide identity with CyHV-3...
متن کاملCurrent knowledge on koi herpesvirus (KHV): a review
The first outbreaks of a disease connected with high mortality of common carp and koi carp caused by koi herpesvirus (KHV) were reported in 1998 in Israel and in the United States. Since then, several cases have been confirmed all over the world. At present, this viral disease is considered to be one of the most risky factors affecting populations of common carp and koi carp. Affected fish beco...
متن کاملDevelopment of mRNA-specific RT-PCR for the detection of koi herpesvirus (KHV) replication stage.
An mRNA-specific reverse transcription (RT)-PCR primer set spanning the exon junction of a spliced putative terminase gene in the koi herpesvirus (KHV) was developed to detect the replicating stage of the virus. The proposed RT-PCR amplified a target gene from the RNA template, but not from a DNA template extracted from common carp brain (CCB) cells infected with KHV. In addition, the RT-PCR di...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2014
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao02784