Detection of koi herpesvirus (KHV) in healthy cyprinid seed stock

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The Current Problem : Koi Herpesvirus (khv)

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Detection of novel strains of cyprinid herpesvirus closely related to koi herpesvirus.

Cyprinid herpesvirus 3 (CyHV-3) or koi herpesvirus (KHV) is a devastating virus of carp. Using generic primers for the DNA polymerase and the major capsid protein genes of cyprinid herpesviruses, nucleotide sequences divergent from previously described CyHV-3 were obtained. At least 3 novel groups of putative CyHV-3-like viruses were identified, sharing 95 to 98% nucleotide identity with CyHV-3...

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An mRNA-specific reverse transcription (RT)-PCR primer set spanning the exon junction of a spliced putative terminase gene in the koi herpesvirus (KHV) was developed to detect the replicating stage of the virus. The proposed RT-PCR amplified a target gene from the RNA template, but not from a DNA template extracted from common carp brain (CCB) cells infected with KHV. In addition, the RT-PCR di...

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ژورنال

عنوان ژورنال: Diseases of Aquatic Organisms

سال: 2014

ISSN: 0177-5103,1616-1580

DOI: 10.3354/dao02784